SkfG

Gene name	skfG
Synonyms	ybdD
Essential	no
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: skfG
MW, pI	14 kDa, 5.186
Gene length, protein length	411 bp, 137 aa
Immediate neighbours	skfF, skfH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteins of unknown function

This gene is a member of the following regulons

AbrB regulon, PhoP regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU01970

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31429

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: skfA-skfB-skfC-skfE-skfF-skfG-skfH PubMed

• Expression browser: skfG PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during logrithmic growth (AbrB) PubMed
  • induced by phosphate starvation (PhoP) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed
  • AbrB: transcription repression PubMed
  • PhoP: transcription activation PubMed

• Additional information: Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfE-skfF-skfG-skfH transcript. PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original Publications

Mark A Strauch, Benjamin G Bobay, John Cavanagh, Fude Yao, Angelo Wilson, Yoann Le Breton
Abh and AbrB control of Bacillus subtilis antimicrobial gene expression.
J Bacteriol: 2007, 189(21);7720-32
[PubMed:17720793] [WorldCat.org] [DOI] (P p)

Nicholas E E Allenby, Carys A Watts, Georg Homuth, Zoltán Prágai, Anil Wipat, Alan C Ward, Colin R Harwood
Phosphate starvation induces the sporulation killing factor of Bacillus subtilis.
J Bacteriol: 2006, 188(14);5299-303
[PubMed:16816204] [WorldCat.org] [DOI] (P p)

Masaya Fujita, José Eduardo González-Pastor, Richard Losick
High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.
J Bacteriol: 2005, 187(4);1357-68
[PubMed:15687200] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

José E González-Pastor, Errett C Hobbs, Richard Losick
Cannibalism by sporulating bacteria.
Science: 2003, 301(5632);510-3
[PubMed:12817086] [WorldCat.org] [DOI] (I p)