YflS

• Description: malate transporter
  
  

Gene name	yflS
Synonyms
Essential	no
Product	malate transporter
Function	malate uptake
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	51 kDa, 9.741
Gene length, protein length	1434 bp, 478 aa
Immediate neighbours	pel, citS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

MalR regulon

The gene

Basic information

• Locus tag: BSU07570

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: View classification (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34726

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yflS PubMed

• Expression browser: yflS PubMed

• Sigma factor:

• Regulation: induced by malate (MalR)

• Regulatory mechanism: MalR: transcription activation

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

• Mutant: GP1450 (cat), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947

Kousei Tanaka, Kazuo Kobayashi, Naotake Ogasawara
The Bacillus subtilis YufLM two-component system regulates the expression of the malate transporters MaeN (YufR) and YflS, and is essential for utilization of malate in minimal medium.
Microbiology (Reading): 2003, 149(Pt 9);2317-2329
[PubMed:12949159] [WorldCat.org] [DOI] (P p)