Difference between revisions of "GabT"
| Line 12: | Line 12: | ||
|style="background:#ABCDEF;" align="center"| '''Product''' || gamma-aminobutyrate transaminase | |style="background:#ABCDEF;" align="center"| '''Product''' || gamma-aminobutyrate transaminase | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || utilization of gamma-amino butyric acid | + | |style="background:#ABCDEF;" align="center"|'''Function''' || utilization of gamma-amino butyric acid and synthesis of norspermidine |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 5.304 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 5.304 | ||
| Line 40: | Line 40: | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[utilization of amino acids]]}} | + | {{SubtiWiki category|[[utilization of amino acids]]}}, |
| + | {{SubtiWiki category|[[miscellaneous metabolic pathways]]}}, | ||
| + | {{SubtiWiki category|[[biofilm formation]]}} | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| Line 52: | Line 54: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | ** block in norspermidine production {{PubMed|22541437}} | ||
| + | ** impaired biofilm disassembly {{PubMed|22541437}} | ||
=== Database entries === | === Database entries === | ||
| Line 68: | Line 72: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate (according to Swiss-Prot) | + | * '''Catalyzed reaction/ biological activity:''' |
| + | ** 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate (according to Swiss-Prot) | ||
* '''Protein family:''' class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot) | * '''Protein family:''' class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot) | ||
| Line 138: | Line 143: | ||
=References= | =References= | ||
| − | <pubmed>15223311,12123465,, </pubmed> | + | <pubmed>15223311,12123465,22541437, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 15:09, 6 May 2012
- Description: gamma-aminobutyrate transaminase, general stress protein
| Gene name | gabT |
| Synonyms | ycnG |
| Essential | no |
| Product | gamma-aminobutyrate transaminase |
| Function | utilization of gamma-amino butyric acid and synthesis of norspermidine |
| MW, pI | 47 kDa, 5.304 |
| Gene length, protein length | 1308 bp, 436 aa |
| Immediate neighbours | gabR, gabD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
utilization of amino acids, miscellaneous metabolic pathways, biofilm formation
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU03900
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate (according to Swiss-Prot)
- Protein family: class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P94427
- KEGG entry: [3]
- E.C. number: 2.6.1.19
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Ilana Kolodkin-Gal, Shugeng Cao, Liraz Chai, Thomas Böttcher, Roberto Kolter, Jon Clardy, Richard Losick
A self-produced trigger for biofilm disassembly that targets exopolysaccharide.
Cell: 2012, 149(3);684-92
[PubMed:22541437]
[WorldCat.org]
[DOI]
(I p)
Boris R Belitsky
Bacillus subtilis GabR, a protein with DNA-binding and aminotransferase domains, is a PLP-dependent transcriptional regulator.
J Mol Biol: 2004, 340(4);655-64
[PubMed:15223311]
[WorldCat.org]
[DOI]
(P p)
Boris R Belitsky, Abraham L Sonenshein
GabR, a member of a novel protein family, regulates the utilization of gamma-aminobutyrate in Bacillus subtilis.
Mol Microbiol: 2002, 45(2);569-83
[PubMed:12123465]
[WorldCat.org]
[DOI]
(P p)
