Difference between revisions of "RnhB"

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=Biological materials =
 
=Biological materials =

Revision as of 08:55, 13 April 2012

  • Description: RNase HII, endoribonuclease

Gene name rnhB
Synonyms rnh
Essential no
Product Mn2+-dependent RNase HII
Function endonucleolytic cleavage of RNA in
RNA-DNA hybrid molecules
MW, pI 28 kDa, 5.518
Gene length, protein length 765 bp, 255 aa
Immediate neighbours rbgA, ylqG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RnhB context.gif
This image was kindly provided by SubtiList




Categories containing this gene/protein

DNA replication, Rnases

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU16060

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)
  • Protein family: RNase HII family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number: 3.1.26.4.

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Takashi Tadokoro, Shigenori Kanaya
Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes.
FEBS J: 2009, 276(6);1482-93
[PubMed:19228197] [WorldCat.org] [DOI] (I p)

Original publications

Sanae Fukushima, Mitsuhiro Itaya, Hiroaki Kato, Naotake Ogasawara, Hirofumi Yoshikawa
Reassessment of the in vivo functions of DNA polymerase I and RNase H in bacterial cell growth.
J Bacteriol: 2007, 189(23);8575-83
[PubMed:17905985] [WorldCat.org] [DOI] (I p)

Mitsuru Haruki, Yasuo Tsunaka, Masaaki Morikawa, Shigenori Kanaya
Cleavage of a DNA-RNA-DNA/DNA chimeric substrate containing a single ribonucleotide at the DNA-RNA junction with prokaryotic RNases HII.
FEBS Lett: 2002, 531(2);204-8
[PubMed:12417313] [WorldCat.org] [DOI] (P p)

M Itaya, A Omori, S Kanaya, R J Crouch, T Tanaka, K Kondo
Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168.
J Bacteriol: 1999, 181(7);2118-23
[PubMed:10094689] [WorldCat.org] [DOI] (P p)

N Ohtani, M Haruki, M Morikawa, R J Crouch, M Itaya, S Kanaya
Identification of the genes encoding Mn2+-dependent RNase HII and Mg2+-dependent RNase HIII from Bacillus subtilis: classification of RNases H into three families.
Biochemistry: 1999, 38(2);605-18
[PubMed:9888800] [WorldCat.org] [DOI] (P p)