Difference between revisions of "Rnc"
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* '''Operon:''' ''[[rnc]]-[[smc]]-[[ftsY]]'' {{PubMed|11021934}} | * '''Operon:''' ''[[rnc]]-[[smc]]-[[ftsY]]'' {{PubMed|11021934}} | ||
| + | |||
| + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rnc_1665710_1666459_1 rnc] {{PubMed|22383849}} | ||
* '''Sigma factor:''' [[SigA]] {{PubMed|11021934}} | * '''Sigma factor:''' [[SigA]] {{PubMed|11021934}} | ||
| Line 114: | Line 116: | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 08:51, 13 April 2012
- Description: RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria
| Gene name | rnc |
| Synonyms | rncS |
| Essential | yes |
| Product | endoribonuclease III |
| Function | cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr) |
| Metabolic function and regulation of this protein in SubtiPathways: Protein secretion | |
| MW, pI | 28 kDa, 8.076 |
| Gene length, protein length | 747 bp, 249 aa |
| Immediate neighbours | acpA, smc |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
Rnases, translation, protein secretion, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU15930
Phenotypes of a mutant
essential PubMed
rnc is essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot) cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P51833
- KEGG entry: [3]
- E.C. number: 3.1.26.3
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
David Bechhofer, Mount Sinai School, New York, USA Homepage
Ciaran Condon, IBPC, Paris, France Homepage
Your additional remarks
References
Reviews
Original publications
Sylvain Durand, Laetitia Gilet, Philippe Bessières, Pierre Nicolas, Ciarán Condon
Three essential ribonucleases-RNase Y, J1, and III-control the abundance of a majority of Bacillus subtilis mRNAs.
PLoS Genet: 2012, 8(3);e1002520
[PubMed:22412379]
[WorldCat.org]
[DOI]
(I p)
Iñigo Lasa, Alejandro Toledo-Arana, Alexander Dobin, Maite Villanueva, Igor Ruiz de los Mozos, Marta Vergara-Irigaray, Víctor Segura, Delphine Fagegaltier, José R Penadés, Jaione Valle, Cristina Solano, Thomas R Gingeras
Genome-wide antisense transcription drives mRNA processing in bacteria.
Proc Natl Acad Sci U S A: 2011, 108(50);20172-7
[PubMed:22123973]
[WorldCat.org]
[DOI]
(I p)
Shiyi Yao, Joshua B Blaustein, David H Bechhofer
Processing of Bacillus subtilis small cytoplasmic RNA: evidence for an additional endonuclease cleavage site.
Nucleic Acids Res: 2007, 35(13);4464-73
[PubMed:17576666]
[WorldCat.org]
[DOI]
(I p)
M A Herskovitz, D H Bechhofer
Endoribonuclease RNase III is essential in Bacillus subtilis.
Mol Microbiol: 2000, 38(5);1027-33
[PubMed:11123676]
[WorldCat.org]
[DOI]
(P p)
Hiroshi Kakeshita, Akihiro Oguro, Reiko Amikura, Kouji Nakamura, Kunio Yamane
Expression of the ftsY gene, encoding a homologue of the alpha subunit of mammalian signal recognition particle receptor, is controlled by different promoters in vegetative and sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 10);2595-2603
[PubMed:11021934]
[WorldCat.org]
[DOI]
(P p)
A Oguro, H Kakeshita, K Nakamura, K Yamane, W Wang, D H Bechhofer
Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor.
J Biol Chem: 1998, 273(31);19542-7
[PubMed:9677377]
[WorldCat.org]
[DOI]
(P p)
W Wang, D H Bechhofer
Bacillus subtilis RNase III gene: cloning, function of the gene in Escherichia coli, and construction of Bacillus subtilis strains with altered rnc loci.
J Bacteriol: 1997, 179(23);7379-85
[PubMed:9393702]
[WorldCat.org]
[DOI]
(P p)
S Mitra, D H Bechhofer
Substrate specificity of an RNase III-like activity from Bacillus subtilis.
J Biol Chem: 1994, 269(50);31450-6
[PubMed:7527387]
[WorldCat.org]
(P p)
A T Panganiban, H R Whiteley
Bacillus subtilis RNAase III cleavage sites in phage SP82 early mRNA.
Cell: 1983, 33(3);907-13
[PubMed:6409421]
[WorldCat.org]
[DOI]
(P p)
