CwlS

• Description: D,L-endopeptidase, peptidoglycan hydrolase
  
  

Gene name	cwlS
Synonyms	yojL
Essential	no
Product	D,L-endopeptidase, peptidoglycan hydrolase
Function	cell wall metabolism
MW, pI	44 kDa, 10.438
Gene length, protein length	1242 bp, 414 aa
Immediate neighbours	yojM, yojK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall degradation/ turnover, membrane proteins

This gene is a member of the following regulons

Abh regulon, AbrB regulon, CcpA regulon, SigD regulon, SigH regulon,

The gene

Basic information

• Locus tag: BSU19410

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of gamma-D-glutamyl bonds to the L-terminus (position 7) of meso-diaminopimelic acid (meso-A₂pm) in 7-(L-Ala-gamma-D-Glu)-meso-A₂pm and 7-(L-Ala-gamma-D-Glu)-7-(D-Ala)-meso-A₂pm (according to Swiss-Prot)

• Protein family: Cu-Zn superoxide dismutase family (according to Swiss-Prot)

• Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity

Extended information on the protein

• Kinetic information:

• Domains:
  • C-terminal D,L-endopeptidase domain PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)
  • localizes to cell septa and poles PubMed

Database entries

• Structure:

• UniProt: O31852

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor: SigD, SigH, according to PubMed

• Regulation:
  • repressed by glucose (3-fold) (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression
  • Abh and AbrB: synergistic transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed