RsbR

• Description: activator of RsbT kinase activity, part of the stressosome
  
  

Gene name	rsbR
Synonyms	ycxR, rsbRA
Essential	no
Product	activator of RsbT kinase activity
Function	control of SigB activity
Interactions involving this protein in SubtInteract: RbsR
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	30 kDa, 4.731
Gene length, protein length	822 bp, 274 aa
Immediate neighbours	ndoA, rsbS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

sigma factors and their control, phosphoproteins

The gene

Basic information

• Locus tag: BSU04670

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): RsbRB, RsbRC, RsbRD, YtvA

Extended information on the protein

• Kinetic information:

• Domains: RsbRA is composed of an N-terminal nonheme globin domain and a highly conserved C-terminal STAS (Sulphate Transporter and AntiSigma factor antagonist) domain. The C-terminal STAS domain is the target of the serine/threonine-specific kinase RsbT (see below).

• Modification: phosphorylation on Thr-171 and Thr-205 by RsbT PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RsbX-RsbR PubMed
  • RsbR-RsbT PubMed, RsbR-RsbS
  • component of the stressosome

• Localization:

Database entries

• Structure: 2BNL (N-terminal domain), 3VY9 (complete stressosome)

• UniProt: P42409

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rsbR-rsbS-rsbT-rsbU-rsbV-rsbW-sigB-rsbX PubMed

• Sigma factor: SigA PubMed

• Regulation: constitutively expressed PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Bill Haldenwang, San Antonio, USA
• Chet Price, Davis, USA homepage
• Rick Lewis, Newcastle, UK homepage

References

Reviews

Jon Marles-Wright, Richard J Lewis
The stressosome: molecular architecture of a signalling hub.
Biochem Soc Trans: 2010, 38(4);928-33
[PubMed:20658979] [WorldCat.org] [DOI] (I p)

Jon Marles-Wright, Richard J Lewis
The Bacillus subtilis stressosome: A signal integration and transduction hub.
Commun Integr Biol: 2008, 1(2);182-4
[PubMed:19704888] [WorldCat.org] [DOI] (I p)

Jan Pané-Farré, Richard J Lewis, Jörg Stülke
The RsbRST stress module in bacteria: a signalling system that may interact with different output modules.
J Mol Microbiol Biotechnol: 2005, 9(2);65-76
[PubMed:16319496] [WorldCat.org] [DOI] (P p)

Original Articles

Additional publications: PubMed