GltC

• Synonyms:

• Description: Transcriptional activator of the gltAB operon. Activates expression of the operon in the absence of arginine.

The gene

Basic information

• Coordinates:

• Gene length:

Database entries

• DBTBS entry: [1]

• SubtiList entry:[2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription activation of the gltAB operon

• Protein family: LysR-type transcription regulator

• Paralogous protein(s): none, but there are 19 members of the LysR family in B. subtilis

Extended information on the protein

• Kinetic information:

• Domains: DNA-binding helix-turn-helix motif: AA 18 ... 37

• Modification:

• Effectors of protein activity: 2-oxoglutarate

• Interactions:
  • GltC-RocG, This interaction takes place in the presence of glutamate. It prevents the transcription activation of the gltAB operon. Note that RocG expression is strongly regulated. <ref>Commichau et al. (2007)</ref>

• Localization:

Database entries

• Structure:

• Swiss prot entry: P20668

• KEGG entry: KEGG

Expression and regulation

• Operon: gltC

• Sigma factor: SigA

• Regulation: autoregulation by GltC

• Regulatory mechanism: autorepression

• Database entries: DBTBS

• Additional information:

Biological materials

• Mutant: GP344 (erm), GP738 (spc) (available in Stülke lab)

• Expression vector: pGP903 (N-terminal His-tag) (available in Stülke lab)

• lacZ fusion:

• GFP fusion:

• Antibody: available in Stülke lab

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA [3]

Jörg Stülke, University of Göttingen, Germany [4]

References

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. [5]