SpoIIIE

• Description: ATP-dependent DNA translocase, transports the forespore chromosome across the sporulation septum
  
  

Gene name	spoIIIE
Synonyms
Essential	no
Product	ATP-dependent DNA translocase required for chromosome partitioning through the septum into the forespore
Function	chromosome partition during sporulation
MW, pI	86 kDa, 6.757
Gene length, protein length	2361 bp, 787 aa
Immediate neighbours	tepA, ymfC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA condensation/ segregation, sporulation/ other, membrane proteins

The gene

Basic information

• Locus tag: BSU16800

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • transports the forespore chromosome across the sporulation septum PubMed
  • translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes PubMed
  • the two DNA translocases SftA and SpoIIIE synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively PubMed

• Protein family:

• Paralogous protein(s): SftA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: transmembrane domain mediates dynamic localization to active division sites PubMed

Database entries

• Structure:

• UniProt: P21458

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIIIE PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Christine Kaimer, Peter L Graumann
Players between the worlds: multifunctional DNA translocases.
Curr Opin Microbiol: 2011, 14(6);719-25
[PubMed:22047950] [WorldCat.org] [DOI] (I p)

Original publications

Christine Kaimer, Katrin Schenk, Peter L Graumann
Two DNA translocases synergistically affect chromosome dimer resolution in Bacillus subtilis.
J Bacteriol: 2011, 193(6);1334-40
[PubMed:21239579] [WorldCat.org] [DOI] (I p)

Tinya C Fleming, Jae Yen Shin, Sang-Hyuk Lee, Eric Becker, Kerwyn Casey Huang, Carlos Bustamante, Kit Pogliano
Dynamic SpoIIIE assembly mediates septal membrane fission during Bacillus subtilis sporulation.
Genes Dev: 2010, 24(11);1160-72
[PubMed:20516200] [WorldCat.org] [DOI] (I p)

David J Sherratt, Lidia K Arciszewska, Estelle Crozat, James E Graham, Ian Grainge
The Escherichia coli DNA translocase FtsK.
Biochem Soc Trans: 2010, 38(2);395-8
[PubMed:20298190] [WorldCat.org] [DOI] (I p)

Christine Kaimer, José Eduardo González-Pastor, Peter L Graumann
SpoIIIE and a novel type of DNA translocase, SftA, couple chromosome segregation with cell division in Bacillus subtilis.
Mol Microbiol: 2009, 74(4);810-25
[PubMed:19818024] [WorldCat.org] [DOI] (I p)

Steven J Biller, William F Burkholder
The Bacillus subtilis SftA (YtpS) and SpoIIIE DNA translocases play distinct roles in growing cells to ensure faithful chromosome partitioning.
Mol Microbiol: 2009, 74(4);790-809
[PubMed:19788545] [WorldCat.org] [DOI] (I p)

Kathleen A Marquis, Briana M Burton, Marcelo Nollmann, Jerod L Ptacin, Carlos Bustamante, Sigal Ben-Yehuda, David Z Rudner
SpoIIIE strips proteins off the DNA during chromosome translocation.
Genes Dev: 2008, 22(13);1786-95
[PubMed:18593879] [WorldCat.org] [DOI] (P p)

Jerod L Ptacin, Marcelo Nollmann, Eric C Becker, Nicholas R Cozzarelli, Kit Pogliano, Carlos Bustamante
Sequence-directed DNA export guides chromosome translocation during sporulation in Bacillus subtilis.
Nat Struct Mol Biol: 2008, 15(5);485-93
[PubMed:18391964] [WorldCat.org] [DOI] (I p)

Briana M Burton, Kathleen A Marquis, Nora L Sullivan, Tom A Rapoport, David Z Rudner
The ATPase SpoIIIE transports DNA across fused septal membranes during sporulation in Bacillus subtilis.
Cell: 2007, 131(7);1301-12
[PubMed:18160039] [WorldCat.org] [DOI] (P p)

Sigal Ben-Yehuda, David Z Rudner, Richard Losick
Assembly of the SpoIIIE DNA translocase depends on chromosome trapping in Bacillus subtilis.
Curr Biol: 2003, 13(24);2196-200
[PubMed:14680637] [WorldCat.org] (P p)

J Bath, L J Wu, J Errington, J C Wang
Role of Bacillus subtilis SpoIIIE in DNA transport across the mother cell-prespore division septum.
Science: 2000, 290(5493);995-7
[PubMed:11062134] [WorldCat.org] [DOI] (P p)

L J Wu, J Errington
Use of asymmetric cell division and spoIIIE mutants to probe chromosome orientation and organization in Bacillus subtilis.
Mol Microbiol: 1998, 27(4);777-86
[PubMed:9515703] [WorldCat.org] [DOI] (P p)

K Pogliano, A E Hofmeister, R Losick
Disappearance of the sigma E transcription factor from the forespore and the SpoIIE phosphatase from the mother cell contributes to establishment of cell-specific gene expression during sporulation in Bacillus subtilis.
J Bacteriol: 1997, 179(10);3331-41
[PubMed:9150232] [WorldCat.org] [DOI] (P p)

L J Wu, J Errington
Septal localization of the SpoIIIE chromosome partitioning protein in Bacillus subtilis.
EMBO J: 1997, 16(8);2161-9
[PubMed:9155041] [WorldCat.org] [DOI] (P p)

M E Sharpe, J Errington
Postseptational chromosome partitioning in bacteria.
Proc Natl Acad Sci U S A: 1995, 92(19);8630-4
[PubMed:7567988] [WorldCat.org] [DOI] (P p)

L J Wu, P J Lewis, R Allmansberger, P M Hauser, J Errington
A conjugation-like mechanism for prespore chromosome partitioning during sporulation in Bacillus subtilis.
Genes Dev: 1995, 9(11);1316-26
[PubMed:7797072] [WorldCat.org] [DOI] (P p)

L J Wu, J Errington
Bacillus subtilis SpoIIIE protein required for DNA segregation during asymmetric cell division.
Science: 1994, 264(5158);572-5
[PubMed:8160014] [WorldCat.org] [DOI] (P p)

D Foulger, J Errington
The role of the sporulation gene spoIIIE in the regulation of prespore-specific gene expression in Bacillus subtilis.
Mol Microbiol: 1989, 3(9);1247-55
[PubMed:2507870] [WorldCat.org] [DOI] (P p)

S Panzer, R Losick, D Sun, P Setlow
Evidence for an additional temporal class of gene expression in the forespore compartment of sporulating Bacillus subtilis.
J Bacteriol: 1989, 171(1);561-4
[PubMed:2492502] [WorldCat.org] [DOI] (P p)

P D Butler, J Mandelstam
Nucleotide sequence of the sporulation operon, spoIIIE, of Bacillus subtilis.
J Gen Microbiol: 1987, 133(9);2359-70
[PubMed:3129532] [WorldCat.org] [DOI] (P p)