Difference between revisions of "GroEL"

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'''Additional publications:''' {{PubMed|22268681}}
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[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 08:57, 25 January 2012

  • Description: chaperonin and co-repressor for HrcA

Gene name groEL
Synonyms
Essential yes PubMed
Product chaperonin, co-repressor for HrcA
Function protein folding and re-folding
Interactions involving this protein in SubtInteract: GroEL
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 57 kDa, 4.531
Gene length, protein length 1632 bp, 544 aa
Immediate neighbours groES, ydiM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GroEL context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

chaperones/ protein folding, heat shock proteins, essential genes, phosphoproteins

This gene is a member of the following regulons

HrcA regulon

The gene

Basic information

  • Locus tag: BSU06030

Expression

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: acts as co-repressor for HrcA
  • Protein family: chaperonin (HSP60) family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on ser/ thr/ tyr PubMed, PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • cytoplasm (according to Swiss-Prot)
    • recruited to the membrane after ethanol stress PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism: HrcA: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1000 available in Stülke lab
    • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP1002 available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage

Your additional remarks

References

Additional publications: PubMed