Difference between revisions of "PGP574"

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<pubmed> 17074746 </pubmed>
 
<pubmed> 17074746 </pubmed>
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* sequencing primer:
 +
**'''T7:''' 5’-TAATACGACTCACTATAGGG -3’
 +
** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’

Revision as of 08:10, 18 January 2012

Vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the C-terminus of the expressed protein

host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Stülke lab

in E. coli: ampicillin resistance

based on pET3C

similar plasmid: pGP172


  • sequencing primer:
    • T7: 5’-TAATACGACTCACTATAGGG -3’
    • NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’