Prs

• Description: phosphoribosylpyrophosphate synthetase, universally conserved protein
  
  

Gene name	prs
Synonyms
Essential	yes PubMed
Product	phosphoribosylpyrophosphate synthetase
Function	phosphoribosylpyrophosphate synthesis (biosynthesis of histidine)
Interactions involving this protein in SubtInteract: Prs
Metabolic function and regulation of this protein in SubtiPathways: Purine synthesis, Nucleotides (regulation), His, Murein recycling
MW, pI	34 kDa, 5.895
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	gcaD, ctc
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, essential genes, universally conserved proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU00510

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)

• Protein family: ribose-phosphate pyrophosphokinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • subject to feedback inhibition by two end products of purine biosynthesis, adenosine 5'-diphosphate (ADP) and guanosine 5'-diphosphate (GDP) PubMed

• Interactions: PurR-Prs

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1IBS

• UniProt: P14193

• KEGG entry: [3]

• E.C. number: 2.7.6.1

Additional information

universally conserved protein

Expression and regulation

• Operon: gcaD-prs-ctc PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Natalia P Zakataeva, Dmitriy V Romanenkov, Victoria S Skripnikova, Maria V Vitushkina, Vitaliy A Livshits, Alexandr D Kivero, Anna E Novikova
Wild-type and feedback-resistant phosphoribosyl pyrophosphate synthetases from Bacillus amyloliquefaciens: purification, characterization, and application to increase purine nucleoside production.
Appl Microbiol Biotechnol: 2012, 93(5);2023-33
[PubMed:22083279] [WorldCat.org] [DOI] (I p)

Shuobo Shi, Tao Chen, Zhigang Zhang, Xun Chen, Xueming Zhao
Transcriptome analysis guided metabolic engineering of Bacillus subtilis for riboflavin production.
Metab Eng: 2009, 11(4-5);243-52
[PubMed:19446032] [WorldCat.org] [DOI] (I p)

Aloke Kumar Bera, Jianghai Zhu, Howard Zalkin, Janet L Smith
Functional dissection of the Bacillus subtilis pur operator site.
J Bacteriol: 2003, 185(14);4099-109
[PubMed:12837784] [WorldCat.org] [DOI] (P p)

Sangita C Sinha, Joseph Krahn, Byung Sik Shin, Diana R Tomchick, Howard Zalkin, Janet L Smith
The purine repressor of Bacillus subtilis: a novel combination of domains adapted for transcription regulation.
J Bacteriol: 2003, 185(14);4087-98
[PubMed:12837783] [WorldCat.org] [DOI] (P p)

I Hilden, B N Krath, B Hove-Jensen
Tricistronic operon expression of the genes gcaD (tms), which encodes N-acetylglucosamine 1-phosphate uridyltransferase, prs, which encodes phosphoribosyl diphosphate synthetase, and ctc in vegetative cells of Bacillus subtilis.
J Bacteriol: 1995, 177(24);7280-4
[PubMed:8522540] [WorldCat.org] [DOI] (P p)

K Arnvig, B Hove-Jensen, R L Switzer
Purification and properties of phosphoribosyl-diphosphate synthetase from Bacillus subtilis.
Eur J Biochem: 1990, 192(1);195-200
[PubMed:2169413] [WorldCat.org] [DOI] (P p)