Difference between revisions of "YxeI"
| Line 1: | Line 1: | ||
| − | * '''Description:''' | + | * '''Description:''' penicillin acylase <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || penicillin acylase |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || resistance to penicillin |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 5.276 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 5.276 | ||
| Line 34: | Line 34: | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[ | + | {{SubtiWiki category|[[Resistance against toxins/ antibiotics]]}} |
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| Line 54: | Line 54: | ||
=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| Line 62: | Line 60: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' acylation of penicillin V {{PubMed|21978958}} |
* '''Protein family:''' peptidase C59 family (according to Swiss-Prot) | * '''Protein family:''' peptidase C59 family (according to Swiss-Prot) | ||
| Line 70: | Line 68: | ||
=== Extended information on the protein === | === Extended information on the protein === | ||
| − | * '''Kinetic information:''' | + | * '''Kinetic information:''' |
| + | ** the apparent K(m) values for penicillin V and the synthetic substrate 2-nitro-5-(phenoxyacetamido)-benzoic acid was 40mM and 0.63mM, respectively {{PubMed|21978958}} | ||
* '''Domains:''' | * '''Domains:''' | ||
| Line 80: | Line 79: | ||
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' | + | * '''[[SubtInteract|Interactions]]:''' |
| + | ** homotetramer {{PubMed|21978958}} | ||
| − | * '''Localization:''' | + | * '''[[Localization]]:''' |
=== Database entries === | === Database entries === | ||
| Line 126: | Line 126: | ||
=References= | =References= | ||
| − | <pubmed>10746760 15937167,, </pubmed> | + | <pubmed>10746760 15937167,21978958, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:50, 8 October 2011
- Description: penicillin acylase
| Gene name | yxeI |
| Synonyms | |
| Essential | no |
| Product | penicillin acylase |
| Function | resistance to penicillin |
| MW, pI | 37 kDa, 5.276 |
| Gene length, protein length | 984 bp, 328 aa |
| Immediate neighbours | yxeJ, yxeH |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
Resistance against toxins/ antibiotics
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU39540
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: acylation of penicillin V PubMed
- Protein family: peptidase C59 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- the apparent K(m) values for penicillin V and the synthetic substrate 2-nitro-5-(phenoxyacetamido)-benzoic acid was 40mM and 0.63mM, respectively PubMed
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- homotetramer PubMed
Database entries
- Structure: 2OQC
- UniProt: P54948
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Priya Rathinaswamy, Sushama M Gaikwad, C G Suresh, Asmita A Prabhune, James A Brannigan, Guy G Dodson, Archana V Pundle
Purification and characterization of YxeI, a penicillin acylase from Bacillus subtilis.
Int J Biol Macromol: 2012, 50(1);25-30
[PubMed:21978958]
[WorldCat.org]
[DOI]
(I p)
Kyle N Erwin, Shunji Nakano, Peter Zuber
Sulfate-dependent repression of genes that function in organosulfur metabolism in Bacillus subtilis requires Spx.
J Bacteriol: 2005, 187(12);4042-9
[PubMed:15937167]
[WorldCat.org]
[DOI]
(P p)
Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760]
[WorldCat.org]
[DOI]
(P p)
