Difference between revisions of "FrlO"

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(Extended information on the protein)
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''[[Interactions]]:''' [[YurJ]]-([[FrlM]]-[[FrlN]])-[[FrlO]] {{PubMed|10092453}}
+
* '''[[SubtInteract|Interactions]]:'''  
 +
** [[YurJ]]-([[FrlM]]-[[FrlN]])-[[FrlO]] {{PubMed|10092453}}
  
* '''Localization:''' membrane associated (via [[FrlM]]-[[FrlN]]) {{PubMed|10092453,18763711}}
+
* '''[[Localization]]:''' membrane associated (via [[FrlM]]-[[FrlN]]) {{PubMed|10092453,18763711}}
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 20:14, 11 August 2011

Gene name frlO
Synonyms yurO
Essential no
Product aminosugar ABC transporter (binding protein)
Function uptake of sugar amines
Interactions involving this protein in SubtInteract: FrlO
Metabolic function and regulation of this protein in SubtiPathways:
Alternative nitrogen sources
MW, pI 47 kDa, 5.674
Gene length, protein length 1266 bp, 422 aa
Immediate neighbours frlN, frlB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YurO context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

ABC transporters, utilization of specific carbon sources, utilization of nitrogen sources other than amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon, FrlR regulon

The gene

Basic information

  • Locus tag: BSU32600

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: bacterial solute-binding protein 1 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • frlB: repressed during growth in the presence of branched chain amino acids (CodY) PubMed
    • frlB: induced in the presence of fructosamine (FrlR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional references: PubMed

Veronika Maria Deppe, Johannes Bongaerts, Timothy O'Connell, Karl-Heinz Maurer, Friedhelm Meinhardt
Enzymatic deglycation of Amadori products in bacteria: mechanisms, occurrence and physiological functions.
Appl Microbiol Biotechnol: 2011, 90(2);399-406
[PubMed:21347729] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)