TsaD

• Description: there are two annotations for the corresponding E. coli protein: O-sialoglycoprotein endopeptidase, and required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA, universally conserved protein
  
  

Gene name	gcp
Synonyms	ydiE
Essential	yes PubMed
Product	O-sialoglycoprotein endopeptidase (putative)
Function	tRNA modification
Interactions involving this protein in SubtInteract: Gcp
MW, pI	36 kDa, 5.016
Gene length, protein length	1038 bp, 346 aa
Immediate neighbours	ydiD, ydiF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

translation, essential genes, universally conserved proteins, poorly characterized/ putative enzymes

The gene

Basic information

• Locus tag: BSU05940

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs PubMed
  • conserved glycopeptidase that may be directly involved in the metabolism of glycated proteins PubMed

• Protein family: peptidase M22 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • the corresponding E. coli protein interacts with the proteins corresponding to YdiB and YdiC PubMed

• Localization:

Database entries

• Structure:

• UniProt: O05518

• KEGG entry: [2]

• E.C. number:

Additional information

universally conserved protein

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: