Blt

• Description: spermidine-efflux transporter
  
  

Gene name	blt
Synonyms	acfA, bmr2, bmt
Essential	no
Product	spermidine-efflux transporter
Function	spermidine export
MW, pI	43 kDa, 10.059
Gene length, protein length	1200 bp, 400 aa
Immediate neighbours	yrkB, bltD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transporters/ other, miscellaneous metabolic pathways

This gene is a member of the following regulons

BltR regulon, Mta regulon

The gene

Basic information

• Locus tag: BSU26590

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: TCR/tet family (according to Swiss-Prot)

• Paralogous protein(s): Bmr

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P39843

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: blt-bltD PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • BltR: transcription activation PubMed
  • Mta: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

D P Woolridge, J D Martinez, D E Stringer, E W Gerner
Characterization of a novel spermidine/spermine acetyltransferase, BltD, from Bacillus subtilis.
Biochem J: 1999, 340 ( Pt 3)(Pt 3);753-8
[PubMed:10359661] [WorldCat.org] (P p)

N N Baranova, A Danchin, A A Neyfakh
Mta, a global MerR-type regulator of the Bacillus subtilis multidrug-efflux transporters.
Mol Microbiol: 1999, 31(5);1549-59
[PubMed:10200972] [WorldCat.org] [DOI] (P p)

D P Woolridge, N Vazquez-Laslop, P N Markham, M S Chevalier, E W Gerner, A A Neyfakh
Efflux of the natural polyamine spermidine facilitated by the Bacillus subtilis multidrug transporter Blt.
J Biol Chem: 1997, 272(14);8864-6
[PubMed:9083003] [WorldCat.org] [DOI] (P p)

M Ahmed, L Lyass, P N Markham, S S Taylor, N Vázquez-Laslop, A A Neyfakh
Two highly similar multidrug transporters of Bacillus subtilis whose expression is differentially regulated.
J Bacteriol: 1995, 177(14);3904-10
[PubMed:7608059] [WorldCat.org] [DOI] (P p)