Difference between revisions of "PcrA"
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' [[YxaL]]-[[PcrA]] {{PubMed|12073041}} | + | * '''[[SubtInteract|Interactions]]:''' |
| + | ** PcRA interacts with the [[RNA polymerase]] {{PubMed|21710567}} | ||
| + | ** [[YxaL]]-[[PcrA]] {{PubMed|12073041}} | ||
| + | ** [[YerB]]-[[PcrA]] {{PubMed|12073041}} | ||
| + | ** [[YwhK]]-[[PcrA]] {{PubMed|12073041}} | ||
* '''Localization:''' | * '''Localization:''' | ||
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<pubmed> 19943906 </pubmed> | <pubmed> 19943906 </pubmed> | ||
==Original Publications== | ==Original Publications== | ||
| + | '''Additional publications:''' {{PubMed|21710567}} | ||
<pubmed>12073041,9701819 ,16267290, 19943900 20723756 18276648 17449621 10899133 10199404 </pubmed> | <pubmed>12073041,9701819 ,16267290, 19943900 20723756 18276648 17449621 10899133 10199404 </pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 19:50, 29 June 2011
- Description: ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer
| Gene name | pcrA |
| Synonyms | yerF |
| Essential | yes PubMed |
| Product | ATP-dependent DNA helicase |
| Function | plasmid rolling-circle replication, conjugative transfer of ICEBs1 |
| MW, pI | 83 kDa, 5.767 |
| Gene length, protein length | 2217 bp, 739 aa |
| Immediate neighbours | pcrB, ligA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
DNA repair/ recombination, mobile genetic elements, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU06610
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: uvrD-like helicase C-terminal domain (according to Swiss-Prot)
- Paralogous protein(s): YjcD
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure: 2PJR (product complex, complex with sulphate ion, Geobacillus stearothermophilus), 1PJR (substrate complex, complex with DNA, Geobacillus stearothermophilus)
- UniProt: O34580
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original Publications
Additional publications: PubMed
Jeehae Park, Sua Myong, Anita Niedziela-Majka, Kyung Suk Lee, Jin Yu, Timothy M Lohman, Taekjip Ha
PcrA helicase dismantles RecA filaments by reeling in DNA in uniform steps.
Cell: 2010, 142(4);544-55
[PubMed:20723756]
[WorldCat.org]
[DOI]
(I p)
Catherine A Lee, Ana Babic, Alan D Grossman
Autonomous plasmid-like replication of a conjugative transposon.
Mol Microbiol: 2010, 75(2);268-79
[PubMed:19943900]
[WorldCat.org]
[DOI]
(I p)
Ye Yang, Shuo-Xing Dou, Hua Ren, Peng-Ye Wang, Xing-Dong Zhang, Min Qian, Bing-Yi Pan, Xu Guang Xi
Evidence for a functional dimeric form of the PcrA helicase in DNA unwinding.
Nucleic Acids Res: 2008, 36(6);1976-89
[PubMed:18276648]
[WorldCat.org]
[DOI]
(I p)
Syam P Anand, Haocheng Zheng, Piero R Bianco, Sanford H Leuba, Saleem A Khan
DNA helicase activity of PcrA is not required for the displacement of RecA protein from DNA or inhibition of RecA-mediated strand exchange.
J Bacteriol: 2007, 189(12);4502-9
[PubMed:17449621]
[WorldCat.org]
[DOI]
(P p)
Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290]
[WorldCat.org]
[DOI]
(P p)
M-F Noirot-Gros, P Soultanas, D B Wigley, S D Ehrlich, P Noirot, M-A Petit
The beta-propeller protein YxaL increases the processivity of the PcrA helicase.
Mol Genet Genomics: 2002, 267(3);391-400
[PubMed:12073041]
[WorldCat.org]
[DOI]
(P p)
P Soultanas, M S Dillingham, P Wiley, M R Webb, D B Wigley
Uncoupling DNA translocation and helicase activity in PcrA: direct evidence for an active mechanism.
EMBO J: 2000, 19(14);3799-810
[PubMed:10899133]
[WorldCat.org]
[DOI]
(P p)
S S Velankar, P Soultanas, M S Dillingham, H S Subramanya, D B Wigley
Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism.
Cell: 1999, 97(1);75-84
[PubMed:10199404]
[WorldCat.org]
[DOI]
(P p)
M A Petit, E Dervyn, M Rose, K D Entian, S McGovern, S D Ehrlich, C Bruand
PcrA is an essential DNA helicase of Bacillus subtilis fulfilling functions both in repair and rolling-circle replication.
Mol Microbiol: 1998, 29(1);261-73
[PubMed:9701819]
[WorldCat.org]
[DOI]
(P p)
