Difference between revisions of "YfmL"
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* '''GFP fusion:''' | * '''GFP fusion:''' | ||
+ | ** B. subtilis GP1081 yfmL-yfp spc, available in [[Stülke]] lab, | ||
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab | * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab |
Revision as of 11:49, 25 March 2011
- Description: DEAD-box RNA helicase
Gene name | yfmL |
Synonyms | |
Essential | no |
Product | DEAD-box RNA helicase |
Function | RNA helicase |
MW, pI | 42 kDa, 9.602 |
Gene length, protein length | 1128 bp, 376 aa |
Immediate neighbours | yfmM, yfmK |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU07430
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: RNA helicase
- Protein family: helicase C-terminal domain (according to Swiss-Prot) DEAD-box RNA helicase
- Paralogous protein(s): CshA, CshB, DeaD
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: O34750
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: yfmL (according to DBTBS)
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- B. subtilis GP1081 yfmL-yfp spc, available in Stülke lab,
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody: