CadA

• Description: cadmium transporting ATPase, resistance to cadmium
  
  

Gene name	cadA
Synonyms	yvgW
Essential	no
Product	cadmium transporting ATPase
Function	cadmium export
Metabolic function and regulation of this protein in SubtiPathways: metal ion homeostasis
MW, pI	75 kDa, 5.998
Gene length, protein length	2106 bp, 702 aa
Immediate neighbours	bdbD, copA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transporters/ other, resistance against toxic metals, membrane proteins

This gene is a member of the following regulons

CzrA regulon

The gene

Basic information

• Locus tag: BSU33490

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + H₂O + Cd²⁺(In) = ADP + phosphate + Cd²⁺(Out) (according to Swiss-Prot)

• Protein family: Type IB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O32219

• KEGG entry: [2]

• E.C. number: 3.6.3.3

Expression and regulation

• Operon: cadA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by toxic metal ions (Zn(II), Cd(II), Co(II), Ni(II) and Cu(II)) (CzrA) PubMed

• Regulatory mechanism:
  • CzrA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

References

Charles M Moore, Ahmed Gaballa, Monica Hui, Rick W Ye, John D Helmann
Genetic and physiological responses of Bacillus subtilis to metal ion stress.
Mol Microbiol: 2005, 57(1);27-40
[PubMed:15948947] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, John D Helmann
Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems.
Biometals: 2003, 16(4);497-505
[PubMed:12779235] [WorldCat.org] [DOI] (P p)

I M Solovieva, K D Entian
Investigation of the yvgW Bacillus subtilis chromosomal gene involved in Cd(2+) ion resistance.
FEMS Microbiol Lett: 2002, 208(1);105-9
[PubMed:11934502] [WorldCat.org] [DOI] (P p)