YwlF

• Description: ribose-5-phosphate isomerase
  
  

Gene name	ywlF
Synonyms	ipc-32d
Essential	no
Product	ribose-5-phosphate isomerase
Function	pentose phosphate pathway
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	16 kDa, 5.416
Gene length, protein length	447 bp, 149 aa
Immediate neighbours	ywlG, ywlE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

• Locus tag: BSU36920

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: lacAB/rpiB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: Reversible Mass Action Kinetics PubMed PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • Inhibited by 6-phosphogluconate and AMP PubMed
  • Inhibited by divalent ions such as Ag2+, Mg2+, Co2+ and Zn2+ PubMed
  • Activated by EDTA and 2-mercaptoethanol PubMed

• Interactions:

• Localization:

Database entries

• Structure: 3HEE (from Clostridium thermocellum, 55% identity, 72% similarity)

• UniProt: P39156

• KEGG entry: [3]

• E.C. number: 5.3.1.6

Additional information

The enzyme has probably more than one active site, but with no cooperativity described PubMed

Expression and regulation

• Operon: ywlF-ywlG PubMed

• Sigma factor:

• Regulation:
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

• Regulatory mechanism:
  • TnrA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

• FLAG-tag construct: GP1404 (spc, based on pGP1331), available in the Stülke lab