Zwf

• Description: glucose 6-phosphate dehydrogenase, pentose-phosphate pathway
  
  

Gene name	zwf
Synonyms	yqjJ
Essential	no
Product	glucose 6-phosphate dehydrogenase
Function	initiation of the pentose phosphate pathway
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	55 kDa, 5.28
Gene length, protein length	1467 bp, 489 aa
Immediate neighbours	rnz, gndA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

carbon core metabolism

The gene

Basic information

• Locus tag: BSU23850

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-glucose 6-phosphate + NADP⁺ = D-glucono-1,5-lactone 6-phosphate + NADPH (according to Swiss-Prot)

• Protein family: glucose-6-phosphate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: Two Substrate Michaelis-Menten PubMed

• Domains:

• Modification:

• Cofactor(s): Mg2+, Mn2+, Ca2+

• Effectors of protein activity:
  • NAD+, NADP+ and NADPH affect the enzyme kinetic PubMed

• Interactions:

• Localization:

Database entries

• Structure: 1DPG (from Leuconostoc Mesenteroides, 45% identity, 62% similarity) PubMed

• UniProt: P54547

• KEGG entry: [3]

• E.C. number: 1.1.1.49

Additional information

The enzyme is a dimer PubMed

Expression and regulation

• Operon: zwf PubMed

• Sigma factor:

• Regulation: constitutive PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • SM-NB3 (zwf-spc), available in Anne Galinier's and Boris Görke's labs

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

• FLAG-tag construct: GP1401 (spc, based on pGP1331), available in the Stülke lab

References

Yun Xia Duan, Tao Chen, Xun Chen, Xue Ming Zhao
Overexpression of glucose-6-phosphate dehydrogenase enhances riboflavin production in Bacillus subtilis.
Appl Microbiol Biotechnol: 2010, 85(6);1907-14
[PubMed:19779711] [WorldCat.org] [DOI] (I p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

P Rowland, A K Basak, S Gover, H R Levy, M J Adams
The three-dimensional structure of glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides refined at 2.0 A resolution.
Structure: 1994, 2(11);1073-87
[PubMed:7881907] [WorldCat.org] [DOI] (P p)

S Ujita, K Kimura
Glucose-6-phosphate dehydrogenase, vegetative and spore Bacillus subtilis.
Methods Enzymol: 1982, 89 Pt D;258-61
[PubMed:6292660] [WorldCat.org] [DOI] (P p)