Difference between revisions of "ProJ"
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=Expression and regulation= | =Expression and regulation= | ||
| − | + | * '''Operon:''' ''[[proH]]-[[proJ]]'' {{PubMed|21296969}} | |
| − | * '''Operon:''' ''[[proH]]-[[proJ]]'' | ||
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
| − | * '''Regulation:''' expressed under conditions of osmotic stress | + | * '''Regulation:''' |
| + | ** expressed under conditions of osmotic stress | ||
| + | ** induced by cold stress {{PubMed|21296969}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
Revision as of 12:39, 8 February 2011
- Description: glutamate 5-kinase
| Gene name | proJ |
| Synonyms | yohA |
| Essential | no |
| Product | glutamate 5-kinase |
| Function | biosynthesis of proline |
| Metabolic function and regulation of this protein in SubtiPathways: Pro, Stress | |
| MW, pI | 40 kDa, 5.296 |
| Gene length, protein length | 1113 bp, 371 aa |
| Immediate neighbours | gltC, proH |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids, coping with hyper-osmotic stress
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU18470
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-glutamate = ADP + L-glutamate 5-phosphate (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s): ProB
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: O07509
- KEGG entry: [2]
- E.C. number: 2.7.2.11
Additional information
Expression and regulation
- Regulation:
- expressed under conditions of osmotic stress
- induced by cold stress PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP817 (spc) available in the Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
