Difference between revisions of "SmpB"
| Line 44: | Line 44: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | + | * impaired growth at high and low temperatures {{PubMed|15805528}} | |
=== Database entries === | === Database entries === | ||
| Line 52: | Line 52: | ||
=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| Line 78: | Line 76: | ||
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' | + | * '''Interactions:''' [[SmpB]]-[[SsrA]] |
* '''Localization:''' cytoplasm (according to Swiss-Prot) | * '''Localization:''' cytoplasm (according to Swiss-Prot) | ||
| Line 97: | Line 95: | ||
* '''Operon:''' | * '''Operon:''' | ||
| + | ** ''[[secG]]-[[yvaK]]-[[rnr]]-[[smpB]]-[[ssrA]]'' {{PubMed|17369301}} | ||
| + | ** ''[[yvaK]]-[[rnr]]-[[smpB]]-[[ssrA]]'' {{PubMed|17369301}} | ||
| + | ** ''[[smpB]]-[[ssrA]]'' {{PubMed|17369301}} | ||
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** ''[[yvaK]]'': [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed] | ||
| − | * ''' | + | * '''Regulation:''' |
| + | ** ''[[yvaK]]'': induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed] | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 128: | Line 131: | ||
=References= | =References= | ||
| − | <pubmed>18977770,11395451,17369301,12730326,,10881189, </pubmed> | + | <pubmed>18977770,11395451,17369301,12730326,,10881189, 15805528 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 07:36, 3 February 2011
- Description: tmRNA-binding protein
| Gene name | smpB |
| Synonyms | yvaI |
| Essential | no |
| Product | tmRNA-binding protein |
| Function | trans-translation |
| MW, pI | 17 kDa, 10.421 |
| Gene length, protein length | 468 bp, 156 aa |
| Immediate neighbours | ssrA, rnr |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU33600
Phenotypes of a mutant
- impaired growth at high and low temperatures PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: smpB family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O32230
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Chet Price, Davis, USA homepage
Your additional remarks
References
Hiromi Ujiie, Tomoko Matsutani, Hisashi Tomatsu, Ai Fujihara, Chisato Ushida, Yasuhiko Miwa, Yasutaro Fujita, Hyouta Himeno, Akira Muto
Trans-translation is involved in the CcpA-dependent tagging and degradation of TreP in Bacillus subtilis.
J Biochem: 2009, 145(1);59-66
[PubMed:18977770]
[WorldCat.org]
[DOI]
(I p)
Ji-Hyun Shin, Chester W Price
The SsrA-SmpB ribosome rescue system is important for growth of Bacillus subtilis at low and high temperatures.
J Bacteriol: 2007, 189(10);3729-37
[PubMed:17369301]
[WorldCat.org]
[DOI]
(P p)
Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528]
[WorldCat.org]
[DOI]
(P p)
Jeffrey H Withey, David I Friedman
A salvage pathway for protein structures: tmRNA and trans-translation.
Annu Rev Microbiol: 2003, 57;101-23
[PubMed:12730326]
[WorldCat.org]
[DOI]
(P p)
T Wiegert, W Schumann
SsrA-mediated tagging in Bacillus subtilis.
J Bacteriol: 2001, 183(13);3885-9
[PubMed:11395451]
[WorldCat.org]
[DOI]
(P p)
A W Karzai, E D Roche, R T Sauer
The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue.
Nat Struct Biol: 2000, 7(6);449-55
[PubMed:10881189]
[WorldCat.org]
[DOI]
(P p)
