Difference between revisions of "SubtInteract"
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Revision as of 12:22, 1 February 2011
Protein-protein interactions are essential for many activities of any living cell. These interactions involve multi-protein complexes that take part in central processes such as DNA replication, transcription or translation. Protein-protein interactions may also be involved in a variety of regulatory events. Metabolic enzymes do often form transien complexes that represent a complete pathways. These complexes are called metabolon. Finally, many interactions may be of a transient nature.
Contents
Methods to detect protein-protein interactions
- Yeast Two Hybrid System PubMed
- TAP-Tag purification PubMed
Attention: Each technique detects only about 33% of all interactions PubMed
Visualization of protein-protein interactions in B. subtilis
- the beta version of an interactive protein-protein interaction map (just replace "PtsH" in the browser line by the protein of your interest)
- the beta version of SubtInteract
Protein complexes in B. subtilis
- DNA replication: the replisome
- transcription: RNA polymerase
- translation: the ribosome
- RNA processing and degradation: the RNA degradosome
- cell division: the divisome
- metabolism: the metabolons of glycolysis and the TCA cycle PubMed
Important publications