IlvB

• Description: acetolactate synthase (large subunit)
  
  

Gene name	ilvB
Synonyms
Essential	no
Product	acetolactate synthase (large subunit)
Function	biosynthesis of branched-chain amino acids
Metabolic function and regulation of this protein in SubtiPathways: Ile, Leu, Val, Coenzyme A
MW, pI	62 kDa, 5.178
Gene length, protein length	1722 bp, 574 aa
Immediate neighbours	ilvH, ysnD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU28310

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

CodY regulon, CcpA regulon, TnrA regulon, T-box

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 pyruvate = 2-acetolactate + CO₂ (according to Swiss-Prot)

• Protein family: TPP enzyme family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1N53 (T box RNA)

• UniProt: P37251

• KEGG entry: [3]

• E.C. number: 2.2.1.6

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD PubMed

• Sigma factor: SigA PubMed

• Regulation: for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
  • expression is stimulated in the presence of glucose (CcpA) PubMed
  • repressed under nitrogen limiting conditions (glutamine or ammonium) (TnrA) PubMed
  • repressed by casamino acids PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • glucose regulation: CcpA PubMed
  • repression by BCAA: tRNA-controlled RNA switch (T-box) that mediates termination/antitermination
  • TnrA: transcription repression PubMed
  • CodY: transcription repression PubMed1 PubMed2

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant: GP324, ilvB under control of pXyl (cat), available in Jörg Stülke lab

• Expression vector:

• lacZ fusion: pGP520 and pGP524 (both in pAC5), available in Stülke lab; pGP523 (in pAC6), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody: