Difference between revisions of "LevB"

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= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[utilization of specific carbon sources]]}},
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{{SubtiWiki category|[[membrane proteins]]}}
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= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[DegU regulon]]}},
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{{SubtiWiki regulon|[[SacY regulon]]}}
  
 
=The gene=
 
=The gene=
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= Categories containing this gene/protein =
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{{SubtiWiki category|[[utilization of specific carbon sources]]}},
 
{{SubtiWiki category|[[membrane proteins]]}}
 
 
=The protein=
 
=The protein=
  

Revision as of 23:39, 8 December 2010

  • Description: endolevanase

Gene name levB
Synonyms yveB
Essential no
Product endolevanase
Function levan degradation
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 58 kDa, 4.993
Gene length, protein length 1548 bp, 516 aa
Immediate neighbours sacB, yveA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YveB context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

DegU regulon, SacY regulon

The gene

Basic information

  • Locus tag: BSU34460

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 32 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induction by sucrose (at high concentration) (SacY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Y Pereira, M F Petit-Glatron, R Chambert
yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis.
Microbiology (Reading): 2001, 147(Pt 12);3413-9
[PubMed:11739774] [WorldCat.org] [DOI] (P p)

S Aymerich, M Steinmetz
Cloning and preliminary characterization of the sacS locus from Bacillus subtilis which controls the regulation of the exoenzyme levansucrase.
Mol Gen Genet: 1987, 208(1-2);114-20
[PubMed:3039303] [WorldCat.org] [DOI] (P p)

H Shimotsu, D J Henner
Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes.
J Bacteriol: 1986, 168(1);380-8
[PubMed:2428811] [WorldCat.org] [DOI] (P p)