Difference between revisions of "MetK"
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| + | = [[Categories]] containing this gene/protein = | ||
| + | {{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}}, | ||
| + | {{SubtiWiki category|[[essential genes]]}} | ||
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| + | = This gene is a member of the following [[regulons]] = | ||
| + | {{SubtiWiki regulon|[[S-box]]}} | ||
=The gene= | =The gene= | ||
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=The protein= | =The protein= | ||
Revision as of 22:41, 8 December 2010
- Description: S-adenosylmethionine synthetase
| Gene name | metK |
| Synonyms | metE |
| Essential | yes PubMed |
| Product | S-adenosylmethionine synthetase |
| Function | S-adenosylmethionine synthesis |
| Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation | |
| MW, pI | 43 kDa, 5.025 |
| Gene length, protein length | 1200 bp, 400 aa |
| Immediate neighbours | asnB, pckA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU30550
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine (according to Swiss-Prot)
- Protein family: AdoMet synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- UniProt: P54419
- KEGG entry: [3]
- E.C. number: 2.5.1.6
Additional information
Expression and regulation
- Regulatory mechanism:
- S-box: riboswitch, binds S-adenosylmethionine resulting in transcription termination PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Brooke A McDaniel, Frank J Grundy, Vineeta P Kurlekar, Jerneja Tomsic, Tina M Henkin
Identification of a mutation in the Bacillus subtilis S-adenosylmethionine synthetase gene that results in derepression of S-box gene expression.
J Bacteriol: 2006, 188(10);3674-81
[PubMed:16672621]
[WorldCat.org]
[DOI]
(P p)
F J Grundy, T M Henkin
The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria.
Mol Microbiol: 1998, 30(4);737-49
[PubMed:10094622]
[WorldCat.org]
[DOI]
(P p)
Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221]
[WorldCat.org]
[DOI]
(P p)
