Difference between revisions of "AlsD"

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(Expression and regulation)
(Expression and regulation)
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Note: since acetate formation requires ''[[ackA]]'' activation by [[CcpA]] there is an indirect effect of [[CcpA]] on the ''alsSD'' operon: the operon is not expressed in ''[[ccpA]]'' mutants
 
Note: since acetate formation requires ''[[ackA]]'' activation by [[CcpA]] there is an indirect effect of [[CcpA]] on the ''alsSD'' operon: the operon is not expressed in ''[[ccpA]]'' mutants
  
* '''Regulatory mechanism:''' [[AlsR]]: repression in the absence of acetoin [http://www.ncbi.nlm.nih.gov/sites/entrez/7685336 PubMed]
+
* '''Regulatory mechanism:''' [[AlsR]]: repression in the absence of acetate [http://www.ncbi.nlm.nih.gov/sites/entrez/7685336 PubMed]
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 15:23, 8 February 2009

  • Description: write here

Gene name alsD
Synonyms
Essential
Product alpha-acetolactate decarboxylase (EC 4.1.1.5)
Function
MW, pI 28 kDa, 4.603
Gene length, protein length 765 bp, 255 aa
Immediate neighbours
Gene sequence (+200bp) Protein sequence
Genetic context
AlsD context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

Note: since acetate formation requires ackA activation by CcpA there is an indirect effect of CcpA on the alsSD operon: the operon is not expressed in ccpA mutants

  • Regulatory mechanism: AlsR: repression in the absence of acetate PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Renna, M. C., Najimudin, N., Winik, L. R., and Zahler, S. A. (1993). Regulation of the Bacillus subtilis alsS, alsD, and alsR genes involved in post-exponential-phase production of acetoin. J. Bacteriol. 175, 3863-3875. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed