PhrA

• Description: response regulator aspartate phosphatase (RapA) inhibitor, control of the phosphorelay
  
  

Gene name	phrA
Synonyms	gsiAB
Essential	no
Product	phosphatase (RapA) inhibitor
Function	control of sporulation initiation
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	4 kDa, 10.229
Gene length, protein length	132 bp, 44 aa
Immediate neighbours	rapA, yjpA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

phosphorelay, quorum sensing, short peptides

This gene is a member of the following regulons

CodY regulon, ComA regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU12440

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: phr family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: RapA-PhrA PubMed

• Localization:

Database entries

• Structure:

• UniProt: Q00829

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapA-phrA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (4.2-fold) (CcpA) PubMed
  • repressed by casamino acids PubMed
  • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • expressed at high cell density (ComA) PubMed

• Regulatory mechanism:
  • ComA: transcription activation (ComA) PubMed
  • CcpA: transcription repression
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: