YurI

• Description: ribonuclease, extracellular RNase Bsn
  
  

Gene name	yurI
Synonyms
Essential	no
Product	extracellular RNase Bsn
Function	extracellular RNA degradation
MW, pI	31 kDa, 4.87
Gene length, protein length	864 bp, 288 aa
Immediate neighbours	pucF, yurJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU32540

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

phosphate metabolism, Rnases

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: O32150

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yurI PubMed

• Sigma factor:

• Regulation:
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Christian Degering, Thorsten Eggert, Michael Puls, Johannes Bongaerts, Stefan Evers, Karl-Heinz Maurer, Karl-Erich Jaeger
Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and heterologous signal peptides.
Appl Environ Microbiol: 2010, 76(19);6370-6
[PubMed:20709850] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Nicholas E E Allenby, Nicola O'Connor, Zoltán Prágai, Alan C Ward, Anil Wipat, Colin R Harwood
Genome-wide transcriptional analysis of the phosphate starvation stimulon of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8063-80
[PubMed:16291680] [WorldCat.org] [DOI] (P p)

Ciarán Condon, Harald Putzer
The phylogenetic distribution of bacterial ribonucleases.
Nucleic Acids Res: 2002, 30(24);5339-46
[PubMed:12490701] [WorldCat.org] [DOI] (I p)

A Nakamura, Y Koide, H Miyazaki, A Kitamura, H Masaki, T Beppu, T Uozumi
Gene cloning and characterization of a novel extracellular ribonuclease of Bacillus subtilis.
Eur J Biochem: 1992, 209(1);121-7
[PubMed:1396690] [WorldCat.org] [DOI] (P p)