SndA

• Description: unknown
  
  

Gene name	ytnL
Synonyms	hipO
Essential	no
Product	unknown
Function	unknown
Metabolic function and regulation of this protein in SubtiPathways: Riboflavin / FAD
MW, pI	45 kDa, 5.545
Gene length, protein length	1248 bp, 416 aa
Immediate neighbours	ytnM, ribR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU29290

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

proteins of unknown function

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: peptidase M20 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34980

• KEGG entry: [3]

• E.C. number:

Additional information

The gene was annotated in KEGG and MetaCyc as hippurate hydrolase EC 3.5.1.32. In Swiss-Prot it is marked as “uncharacterized hydrolase”. No literature/experimental evidence supporting ththeannotation was removed as of November 2009. PubMed

Expression and regulation

• Operon: ytmI-tcyJ-tcyK-tcyL-tcyM-tcyN-ytmO-ytnI-ytnJ-ribR-ytnL-ytnM PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of cysteine (CymR) PubMed

• Regulatory mechanism:
  • CymR: transcription repression PubMed
  • YtlI: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Pierre Burguière, Juliette Fert, Isabelle Guillouard, Sandrine Auger, Antoine Danchin, Isabelle Martin-Verstraete
Regulation of the Bacillus subtilis ytmI operon, involved in sulfur metabolism.
J Bacteriol: 2005, 187(17);6019-30
[PubMed:16109943] [WorldCat.org] [DOI] (P p)

Irina M Solovieva, Rimma A Kreneva, Lubov Errais Lopes, Daniel A Perumov
The riboflavin kinase encoding gene ribR of Bacillus subtilis is a part of a 10 kb operon, which is negatively regulated by the yrzC gene product.
FEMS Microbiol Lett: 2005, 243(1);51-8
[PubMed:15668000] [WorldCat.org] [DOI] (P p)

I M Solov'eva, R A Kreneva, L L Erraĭs, A S Mironov, D A Perumov
[Study of the mechanism for regulating ribR gene activity in Bacillus subtilis]. [Issledovanie mekhanizma reguliatsii aktivnosti gena ribR u Bacillus subtilis.]
Genetika: 2004, 40(5);716-20
[PubMed:15272571] [WorldCat.org] (P p)