RnhC

• Description: RNase HIII, endoribonuclease
  
  

Gene name	rnhC
Synonyms	ysgB
Essential	no
Product	Mg2+-dependent RNase HIII
Function	endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules
MW, pI	33 kDa, 10.07
Gene length, protein length	939 bp, 313 aa
Immediate neighbours	zapA, pheT
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU28620

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

Rnases

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)

• Protein family: RnhC subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2D0B (complex with Mg2+, Geobacillus stearothermophilus , 47% identity), 2D0A (Geobacillus stearothermophilus, 47% identity)

• UniProt: P94541

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rnhC PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Takashi Tadokoro, Shigenori Kanaya
Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes.
FEBS J: 2009, 276(6);1482-93
[PubMed:19228197] [WorldCat.org] [DOI] (I p)

Original publications

Sanae Fukushima, Mitsuhiro Itaya, Hiroaki Kato, Naotake Ogasawara, Hirofumi Yoshikawa
Reassessment of the in vivo functions of DNA polymerase I and RNase H in bacterial cell growth.
J Bacteriol: 2007, 189(23);8575-83
[PubMed:17905985] [WorldCat.org] [DOI] (I p)

M Itaya, A Omori, S Kanaya, R J Crouch, T Tanaka, K Kondo
Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168.
J Bacteriol: 1999, 181(7);2118-23
[PubMed:10094689] [WorldCat.org] [DOI] (P p)

N Ohtani, M Haruki, M Morikawa, R J Crouch, M Itaya, S Kanaya
Identification of the genes encoding Mn2+-dependent RNase HII and Mg2+-dependent RNase HIII from Bacillus subtilis: classification of RNases H into three families.
Biochemistry: 1999, 38(2);605-18
[PubMed:9888800] [WorldCat.org] [DOI] (P p)

A Wipat, N Carter, S C Brignell, B J Guy, K Piper, J Sanders, P T Emmerson, C R Harwood
The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism.
Microbiology (Reading): 1996, 142 ( Pt 11);3067-78
[PubMed:8969504] [WorldCat.org] [DOI] (P p)