Difference between revisions of "LysC"

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= Categories containing this gene/protein =
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{{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}}
 
=The protein=
 
=The protein=
  

Revision as of 17:45, 30 November 2010

  • Description: aspartokinase II (alpha and beta subunits)

Gene name lysC
Synonyms ask, aecA
Essential no
Product aspartokinase II (alpha and beta subunits)
Function biosynthesis of lysine
Metabolic function and regulation of this protein in SubtiPathways:
Lys, Thr
MW, pI 43 kDa, 4.643
Gene length, protein length 1224 bp, 408 aa
Immediate neighbours yslB, ask
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LysC context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU28470

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-aspartate = ADP + 4-phospho-L-aspartate (according to Swiss-Prot)
  • Protein family: aspartokinase family (according to Swiss-Prot)
  • Paralogous protein(s): DapG

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 2RE1 (from Neisseria meningitidis mc58, 40% identity, 58% similarity)
  • KEGG entry: [3]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed, also degraded upon ammonium or amino acid starvation PubMed

Expression and regulation

  • Regulation:
    • repressed in the presence of lysine (L-box) PubMed
    • expression activated by glucose (5.4 fold) PubMed
    • repressed by casamino acids PubMed
  • Regulatory mechanism:
    • L-box: a riboswich that mediates transcription terimnation antitermination control PubMed
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed, also degraded upon ammonium or amino acid starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Chien-Chi Lo, Carol A Bonner, Gary Xie, Mark D'Souza, Roy A Jensen
Cohesion group approach for evolutionary analysis of aspartokinase, an enzyme that feeds a branched network of many biochemical pathways.
Microbiol Mol Biol Rev: 2009, 73(4);594-651
[PubMed:19946135] [WorldCat.org] [DOI] (I p)

Orginal Publications