HxlB

• Description: 6-phospho-3-hexuloisomerase
  
  

Gene name	hxlB
Synonyms	yckF
Essential	no
Product	6-phospho-3-hexuloisomerase
Function	ribulose monophosphate pathway for formaldehyde fixation
MW, pI	19 kDa, 5.791
Gene length, protein length	555 bp, 185 aa
Immediate neighbours	tlpC, hxlA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

resistance against oxidative and electrophile stress

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-arabino-hex-3-ulose 6-phosphate = D-fructose 6-phosphate (according to Swiss-Prot)

• Protein family: PHI subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1VIV

• UniProt: P42404

• KEGG entry: [3]

• E.C. number: 5.3.1.27

Expression and regulation

• Operon: hxlA-hxlB PubMed

• Sigma factor:

• Regulation: induction by formaldehyde (HxlR) PubMed

• Regulatory mechanism: transcription activation by HxlR PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

E J Taylor, S J Charnock, J Colby, G J Davies, G W Black
Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis.
Acta Crystallogr D Biol Crystallogr: 2001, 57(Pt 8);1138-40
[PubMed:11468398] [WorldCat.org] [DOI] (P p)

H Yasueda, Y Kawahara, S Sugimoto
Bacillus subtilis yckG and yckF encode two key enzymes of the ribulose monophosphate pathway used by methylotrophs, and yckH is required for their expression.
J Bacteriol: 1999, 181(23);7154-60
[PubMed:10572115] [WorldCat.org] [DOI] (P p)