GtaB

• Description: UTP-glucose-1-phosphate uridylyltransferase, general stress protein
  
  

Gene name	gtaB
Synonyms
Essential	no
Product	UTP-glucose-1-phosphate uridylyltransferase
Function	biosynthesis of teichoic acid
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	32 kDa, 4.913
Gene length, protein length	876 bp, 292 aa
Immediate neighbours	mnaA, ggaB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU35670

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

cell wall synthesis, lipid metabolism/ other, biosynthesis of cell wall components, general stress proteins (controlled by SigB), phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose (according to Swiss-Prot)

• Protein family: UDPGP type 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2E3D (from E. coli, 44% identity, 61% similarity) PubMed

• UniProt: Q05852

• KEGG entry: [3]

• E.C. number: 2.7.7.9

Expression and regulation

• Operon: gtaB PubMed

• Sigma factor: SigA PubMed, SigB PubMed

• Regulation:
  • induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alain Lévine, Françoise Vannier, Cédric Absalon, Lauriane Kuhn, Peter Jackson, Elaine Scrivener, Valérie Labas, Joëlle Vinh, Patrick Courtney, Jérôme Garin, Simone J Séror
Analysis of the dynamic Bacillus subtilis Ser/Thr/Tyr phosphoproteome implicated in a wide variety of cellular processes.
Proteomics: 2006, 6(7);2157-73
[PubMed:16493705] [WorldCat.org] [DOI] (P p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

D Varón, S A Boylan, K Okamoto, C W Price
Bacillus subtilis gtaB encodes UDP-glucose pyrophosphorylase and is controlled by stationary-phase transcription factor sigma B.
J Bacteriol: 1993, 175(13);3964-71
[PubMed:8320212] [WorldCat.org] [DOI] (P p)

H M Pooley, D Paschoud, D Karamata
The gtaB marker in Bacillus subtilis 168 is associated with a deficiency in UDPglucose pyrophosphorylase.
J Gen Microbiol: 1987, 133(12);3481-93
[PubMed:2846750] [WorldCat.org] [DOI] (P p)