AdaA

• Description: trigger enzyme: methylphosphotriester-DNA alkyltransferase/ transcriptional activator of the adaA-adaB operon
  
  

Gene name	adaA
Synonyms
Essential	no
Product	trigger enzyme: methylphosphotriester-DNA alkyltransferase/ transcriptional activator of the adaAB operon
Function	adaptive response to alkylative DNA damage
MW, pI	24 kDa, 9.185
Gene length, protein length	633 bp, 211 aa
Immediate neighbours	alkA, adaB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU01810

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

DNA repair/ recombination, trigger enzyme

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P19219

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: adaA-adaB PubMed

• Sigma factor: SigA PubMed

• Regulation: positive control by AdaA PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

F Morohoshi, K Hayashi, N Munkata
Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon.
J Bacteriol: 1993, 175(18);6010-7
[PubMed:8376346] [WorldCat.org] [DOI] (P p)

F Morohoshi, K Hayashi, N Munakata
Bacillus subtilis ada operon encodes two DNA alkyltransferases.
Nucleic Acids Res: 1990, 18(18);5473-80
[PubMed:2120677] [WorldCat.org] [DOI] (P p)