Difference between revisions of "PyrG"

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* '''Description:''' CTP synthase (NH3, glutamine) <br/><br/>
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* '''Description:''' CTP synthase (NH3, glutamine) &lt;br/>&lt;br/>
 
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"|'''Function''' || pyrimidine biosynthesis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || pyrimidine biosynthesis
 
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: &lt;br/>[http://subtiwiki.uni-goettingen.de/pathways/pyrimidines_metabolism.html Pyrimidines], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/pyrimidines_metabolism.html Pyrimidines], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 59 kDa, 5.16   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 59 kDa, 5.16   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywjG]]'', ''[[rpoE]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywjG]]'', ''[[rpoE]]''
 
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;#91;EMBLCDS:CAB15743&amp;#93;+-newId sequences] &lt;br/> (Barbe ''et al.'', 2009)'''
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15743&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
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|colspan="2" | '''Genetic context''' &lt;br/> [[Image:pyrG_context.gif]]
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|colspan="2" | '''Genetic context''' <br/> [[Image:pyrG_context.gif]]
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  &lt;div align="right"> &lt;small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&lt;/small>&lt;/div>
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  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
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__TOC__
 
__TOC__
  
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&lt;br/>&lt;br/>&lt;br/>&lt;br/>&lt;br/>&lt;br/>
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<br/><br/><br/><br/><br/><br/>
  
 
=The gene=
 
=The gene=
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
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* '''Catalyzed reaction/ biological activity:''' ATP + UTP + NH&lt;sub>3&lt;/sub> = ADP + phosphate + CTP (according to Swiss-Prot)  
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* '''Catalyzed reaction/ biological activity:''' ATP + UTP + NH<sub>3</sub> = ADP + phosphate + CTP (according to Swiss-Prot)  
  
 
* '''Protein family:''' CTP synthase family (according to Swiss-Prot)
 
* '''Protein family:''' CTP synthase family (according to Swiss-Prot)
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=References=
 
=References=
  
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&lt;pubmed>8206849,17302819,15252202,11544212,1709162,12426364,17158658,2457578  &lt;/pubmed>
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<pubmed>8206849,17302819,15252202,11544212,1709162,12426364,17158658,2457578  </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 05:13, 24 November 2010

  • Description: CTP synthase (NH3, glutamine)

Gene name pyrG
Synonyms ctrA
Essential yes PubMed
Product CTP synthase (NH3, glutamine)
Function pyrimidine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Pyrimidines, Nucleotides (regulation)
MW, pI 59 kDa, 5.16
Gene length, protein length 1605 bp, 535 aa
Immediate neighbours ywjG, rpoE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PyrG context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU37150

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + UTP + NH3 = ADP + phosphate + CTP (according to Swiss-Prot)
  • Protein family: CTP synthase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1VCM (from Thermus thermophilus, 52% identity, 70% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
    • RNA switch: transcription (termination in the presence of cytidine nucleotides) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Irene E Jensen-MacAllister, Qi Meng, Robert L Switzer
Regulation of pyrG expression in Bacillus subtilis: CTP-regulated antitermination and reiterative transcription with pyrG templates in vitro.
Mol Microbiol: 2007, 63(5);1440-52
[PubMed:17302819] [WorldCat.org] [DOI] (P p)

Alexander K W Elsholz, Casper Møller Jørgensen, Robert L Switzer
The number of G residues in the Bacillus subtilis pyrG initially transcribed region governs reiterative transcription-mediated regulation.
J Bacteriol: 2007, 189(5);2176-80
[PubMed:17158658] [WorldCat.org] [DOI] (P p)

Qi Meng, Charles L Turnbough, Robert L Switzer
Attenuation control of pyrG expression in Bacillus subtilis is mediated by CTP-sensitive reiterative transcription.
Proc Natl Acad Sci U S A: 2004, 101(30);10943-8
[PubMed:15252202] [WorldCat.org] [DOI] (P p)

Qi Meng, Robert L Switzer
cis-acting sequences of Bacillus subtilis pyrG mRNA essential for regulation by antitermination.
J Bacteriol: 2002, 184(23);6734-8
[PubMed:12426364] [WorldCat.org] [DOI] (P p)

Q Meng, R L Switzer
Regulation of transcription of the Bacillus subtilis pyrG gene, encoding cytidine triphosphate synthetase.
J Bacteriol: 2001, 183(19);5513-22
[PubMed:11544212] [WorldCat.org] [DOI] (P p)

R J Turner, Y Lu, R L Switzer
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.
J Bacteriol: 1994, 176(12);3708-22
[PubMed:8206849] [WorldCat.org] [DOI] (P p)

C L Quinn, B T Stephenson, R L Switzer
Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.
J Biol Chem: 1991, 266(14);9113-27
[PubMed:1709162] [WorldCat.org] (P p)

K Trach, J W Chapman, P Piggot, D LeCoq, J A Hoch
Complete sequence and transcriptional analysis of the spo0F region of the Bacillus subtilis chromosome.
J Bacteriol: 1988, 170(9);4194-208
[PubMed:2457578] [WorldCat.org] [DOI] (P p)

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