MutY

• Description: A/G-specific adenine glycosylase, DNA glycosylase, releases adenines from 8-oxo-G:A mismatches
  
  

Gene name	yfhQ
Synonyms
Essential	no
Product	error prevention oxidized guanine system
Function	DNA repair
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	41 kDa, 6.044
Gene length, protein length	1107 bp, 369 aa
Immediate neighbours	yfhP, yfhS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU08630

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1RRS (complex with DNA, Geobacillus stearothermophilus)

• UniProt: O31584

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yfhQ-fabL-sspE PubMed

• Sigma factor:
  • SigA PubMed
  • SigG PubMed

• Regulation:
  • YfhP may act as a negative transcription regulator of the yfhQ-fabL-sspE operon during exponential growth PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Bernardo N Debora, Luz E Vidales, Rosario Ramírez, Mariana Ramírez, Eduardo A Robleto, Ronald E Yasbin, Mario Pedraza-Reyes
Mismatch repair modulation of MutY activity drives Bacillus subtilis stationary-phase mutagenesis.
J Bacteriol: 2011, 193(1);236-45
[PubMed:20971907] [WorldCat.org] [DOI] (I p)

Luz E Vidales, Lluvia C Cárdenas, Eduardo Robleto, Ronald E Yasbin, Mario Pedraza-Reyes
Defects in the error prevention oxidized guanine system potentiate stationary-phase mutagenesis in Bacillus subtilis.
J Bacteriol: 2009, 191(2);506-13
[PubMed:19011023] [WorldCat.org] [DOI] (I p)

Hiroki Yamamoto, Masao Mori, Junichi Sekiguchi
Transcription of genes near the sspE locus of the Bacillus subtilis genome.
Microbiology (Reading): 1999, 145 ( Pt 8);2171-2180
[PubMed:10463184] [WorldCat.org] [DOI] (P p)