Difference between revisions of "IlvH"
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* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1577690 PubMed] | * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1577690 PubMed] | ||
| − | * '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | + | * '''Regulation:''' for a complete overview on the regulation of the ilv operon, see [http://www.ncbi.nlm.nih.gov/pubmed/20935095 Brinsmade ''et al''.] |
| + | ** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | ||
| + | ** expressed in the absence of branched-chain amino acids (BCAA) | ||
| + | ** expression is stimulated in the presence of glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/12193635 PubMed] | ||
** repressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15547269 PubMed] | ** repressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15547269 PubMed] | ||
** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ** repressed during growth in the presence of branched chain amino acids ([[CodY]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ||
| − | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' |
| + | ** glucose regulation: [[CcpA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12193635 PubMed] | ||
| + | ** repression by BCAA: tRNA-controlled RNA switch (T-box) that mediates termination/antitermination | ||
** [[TnrA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15547269 PubMed] | ** [[TnrA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15547269 PubMed] | ||
** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12618455 PubMed] | ||
| + | |||
* '''Additional information:''' | * '''Additional information:''' | ||
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=References= | =References= | ||
| − | <pubmed>15060025,12193635,19258532,8289305,18641142,15547269,12618455,,15547269,12618455,12107147, </pubmed> | + | <pubmed>15060025,12193635,19258532,8289305,18641142,15547269,12618455,,15547269,12618455,12107147, 20935095</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:36, 26 October 2010
- Description: acetolactate synthase (large subunit)
| Gene name | ilvH |
| Synonyms | ilvN |
| Essential | no |
| Product | acetolactate synthase (large subunit) |
| Function | biosynthesis of branched-chain amino acids |
| Metabolic function and regulation of this protein in SubtiPathways: Ile, Leu, Val, Coenzyme A | |
| MW, pI | 19 kDa, 10.295 |
| Gene length, protein length | 522 bp, 174 aa |
| Immediate neighbours | ilvC, ilvB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU28300
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2 pyruvate = 2-acetolactate + CO2 (according to Swiss-Prot)
- Protein family: acetolactate synthase small subunit family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- UniProt: P37252
- KEGG entry: [3]
- E.C. number: 2.2.1.6
Additional information
Expression and regulation
- Regulation: for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
- repressed by casamino acids PubMed
- expressed in the absence of branched-chain amino acids (BCAA)
- expression is stimulated in the presence of glucose PubMed
- repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
- repressed during growth in the presence of branched chain amino acids (CodY) PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
