SigA

• Description: RNA polymerase major sigma factor SigA
  
  

Gene name	sigA
Synonyms	rpoD, crsA
Essential	yes PubMed
Product	RNA polymerase major sigma factor SigA
Function	transcription
MW, pI	42 kDa, 4.634
Gene length, protein length	1113 bp, 371 aa
Immediate neighbours	cccA, antE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU25200

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• List of SigA controlled genes

• Protein family: sigma-70 factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: Spo0A-SigA PubMed, SigA-(RpoB-RpoC) PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P06224

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • yqxD-dnaG-sigA PubMed
  • sigA PubMed

• Sigma factor:
  • yqxD: SigA PubMed, SigBPubMed, SigH PubMed
  • sigA: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Charles Moran, Emory University, NC, USA homepage

References

Hsin-Yi Yeh, Tsung-Ching Chen, Kung-Ming Liou, Hsiu-Ting Hsu, Kuei-Min Chung, Li-Ling Hsu, Ban-Yang Chang
The core-independent promoter-specific interaction of primary sigma factor.
Nucleic Acids Res: 2011, 39(3);913-25
[PubMed:20935043] [WorldCat.org] [DOI] (I p)

Elecia B Johnston, Peter J Lewis, Renate Griffith
The interaction of Bacillus subtilis sigmaA with RNA polymerase.
Protein Sci: 2009, 18(11);2287-97
[PubMed:19735077] [WorldCat.org] [DOI] (I p)

Steve D Seredick, George B Spiegelman
The Bacillus subtilis response regulator Spo0A stimulates sigmaA-dependent transcription prior to the major energetic barrier.
J Biol Chem: 2004, 279(17);17397-403
[PubMed:14976210] [WorldCat.org] [DOI] (P p)

Amrita Kumar, Cindy Buckner Starke, Mark DeZalia, Charles P Moran
Surfaces of Spo0A and RNA polymerase sigma factor A that interact at the spoIIG promoter in Bacillus subtilis.
J Bacteriol: 2004, 186(1);200-6
[PubMed:14679239] [WorldCat.org] [DOI] (P p)

Additional publication: PubMed