CsfB

• Description: forespore-specific protein, inhibitor of SigG and of SigE
  
  

Gene name	csfB
Synonyms	yaaM
Essential	no
Product	inhibitor of SigG and SigE
Function	control of SigG and SigE activity
MW, pI	7 kDa, 7.671
Gene length, protein length	192 bp, 64 aa
Immediate neighbours	rrnA-5S, xpaC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00240

Phenotypes of a mutant

premature activation of SigG PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: inhibitor of SigG PubMed1 PubMed2

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: CsfB-SigG PubMed

• Localization: forespore

Database entries

• Structure:

• UniProt: P37534

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: csfB

• Sigma factor: SigF PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Vasant K Chary, Panagiotis Xenopoulos, Avigdor Eldar, Patrick J Piggot
Loss of compartmentalization of σ(E) activity need not prevent formation of spores by Bacillus subtilis.
J Bacteriol: 2010, 192(21);5616-24
[PubMed:20802044] [WorldCat.org] [DOI] (I p)

Lamya Rhayat, Sandra Duperrier, Rut Carballido-López, Olivier Pellegrini, Patrick Stragier
Genetic dissection of an inhibitor of the sporulation sigma factor sigma(G).
J Mol Biol: 2009, 390(5);835-44
[PubMed:19497328] [WorldCat.org] [DOI] (I p)

Vasant K Chary, Panagiotis Xenopoulos, Patrick J Piggot
Expression of the sigmaF-directed csfB locus prevents premature appearance of sigmaG activity during sporulation of Bacillus subtilis.
J Bacteriol: 2007, 189(23);8754-7
[PubMed:17921305] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

A Decatur, R Losick
Identification of additional genes under the control of the transcription factor sigma F of Bacillus subtilis.
J Bacteriol: 1996, 178(16);5039-41
[PubMed:8759874] [WorldCat.org] [DOI] (P p)

C D Webb, A Decatur, A Teleman, R Losick
Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis.
J Bacteriol: 1995, 177(20);5906-11
[PubMed:7592342] [WorldCat.org] [DOI] (P p)