Difference between revisions of "YngI"

From SubtiWiki
Jump to: navigation, search
Line 1: Line 1:
* '''Description:''' aceto-acetate-CoA ligase <br/><br/>
+
* '''Description:''' aceto-acetyl-CoA ligase, may be involved in surfactin production  <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
Line 10: Line 10:
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || aceto-acetate-CoA ligase  
+
|style="background:#ABCDEF;" align="center"| '''Product''' || aceto-acetyl-CoA ligase  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || mother cell metabolism, leucine utilization
 
|style="background:#ABCDEF;" align="center"|'''Function''' || mother cell metabolism, leucine utilization
Line 56: Line 56:
 
* '''Protein family:'''
 
* '''Protein family:'''
  
* '''Paralogous protein(s):'''
+
* '''Paralogous protein(s):''' [[LcfA]], [[LcfB]], [[YhfT]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 101: Line 101:
 
=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
+
* '''Mutant:''' available in [[Mohamed Marahiel]]'s lab {{PubMed|20797616}}
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
Line 118: Line 118:
  
 
=References=
 
=References=
<pubmed>15699190,12662922 19935659 </pubmed>
+
<pubmed>15699190,12662922 19935659 20797616</pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:21, 1 September 2010

  • Description: aceto-acetyl-CoA ligase, may be involved in surfactin production

Gene name yngI
Synonyms
Essential no
Product aceto-acetyl-CoA ligase
Function mother cell metabolism, leucine utilization
MW, pI 61 kDa, 5.978
Gene length, protein length 1647 bp, 549 aa
Immediate neighbours yngH, yngJ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YngI context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + acetoacetate + CoA <=> AMP + diphosphate + acetoacetyl-CoA PubMed
  • Protein family:

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed during sporulation in the mother cell (SigE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Femke I Kraas, Verena Helmetag, Melanie Wittmann, Matthias Strieker, Mohamed A Marahiel
Functional dissection of surfactin synthetase initiation module reveals insights into the mechanism of lipoinitiation.
Chem Biol: 2010, 17(8);872-80
[PubMed:20797616] [WorldCat.org] [DOI] (I p)

Tzu-Lin Hsiao, Olga Revelles, Lifeng Chen, Uwe Sauer, Dennis Vitkup
Automatic policing of biochemical annotations using genomic correlations.
Nat Chem Biol: 2010, 6(1);34-40
[PubMed:19935659] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)