RpoE

• Description: RNA polymerase delta subunit
  
  

Gene name	rpoE
Synonyms
Essential	no
Product	RNA polymerase delta subunit
Function	transcription
Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation
MW, pI	20 kDa, 3.654
Gene length, protein length	519 bp, 173 aa
Immediate neighbours	pyrG, acdA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU37160

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: rpoE family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: closely associated to RNA polymerase (RpoA(2)-RpoB-RpoC) PubMed

• Localization:closely associated with RNA polymerase involved in transcribing both mRNA and rRNA operons PubMed

Database entries

• Structure: 2KRC PubMed

• UniProt: P12464

• KEGG entry: [3]

• E.C. number: 2.7.7.6

Expression and regulation

• Operon:
  • fadF-acdA-rpoE PubMed
  • rpoE PubMed

• Sigma factor:
  • fadF: SigA PubMed
  • rpoE: SigA PubMed

• Regulation:
  • fadF: repressed in the absence of long-chain fatty acids (FadR) PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed

• Additional information:
  • present at equimolar levels with RNA polymerase PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Arthur Aronson, Purdue University, West Lafayette, USA homepage