Difference between revisions of "NadF"
| Line 70: | Line 70: | ||
* '''Cofactor(s):''' | * '''Cofactor(s):''' | ||
| − | * '''Effectors of protein activity:''' | + | * '''Effectors of protein activity:''' allosterically activated by quinolinic acid |
* '''Interactions:''' | * '''Interactions:''' | ||
| Line 89: | Line 89: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | + | * '''Operon:''' ''[[yjbL]]-[[yjbM]]-[[nadF]]-[[yjbO]]'' {{PubMed|18067544}} | |
| − | * '''Operon:''' | ||
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
| − | * '''Regulation:''' | + | * '''Regulation:''' expressed during exponential growth |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| Line 120: | Line 119: | ||
=References= | =References= | ||
| − | <pubmed>12897004,, </pubmed> | + | <pubmed>12897004,18067544, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 20:44, 5 June 2010
- Description: NAD kinase
| Gene name | nadF |
| Synonyms | yjbN, ppnK |
| Essential | yes PubMed |
| Product | inorganic polyphosphate/ATP-NAD kinase |
| Function | generation of NADP from NAD |
| MW, pI | 29 kDa, 5.991 |
| Gene length, protein length | 798 bp, 266 aa |
| Immediate neighbours | yjbM, yjbO |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU11610
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + NAD+ = ADP + NADP+ (according to Swiss-Prot)
- Protein family: NAD kinase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity: allosterically activated by quinolinic acid
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O31612
- KEGG entry: [2]
- E.C. number: 2.7.1.23
Additional information
Expression and regulation
- Regulation: expressed during exponential growth
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Hideaki Nanamiya, Koji Kasai, Akira Nozawa, Choong-Soo Yun, Takakuni Narisawa, Kana Murakami, Yousuke Natori, Fujio Kawamura, Yuzuru Tozawa
Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis.
Mol Microbiol: 2008, 67(2);291-304
[PubMed:18067544]
[WorldCat.org]
[DOI]
(P p)
Silvia Garavaglia, Alessandro Galizzi, Menico Rizzi
Allosteric regulation of Bacillus subtilis NAD kinase by quinolinic acid.
J Bacteriol: 2003, 185(16);4844-50
[PubMed:12897004]
[WorldCat.org]
[DOI]
(P p)
