YflG

• Description: methionine aminopeptidase
  
  

Gene name	yflG
Synonyms
Essential	no
Product	methionine aminopeptidase
Function	removal of N-terminal methionine from nascent proteins
MW, pI	27 kDa, 5.578
Gene length, protein length	747 bp, 249 aa
Immediate neighbours	yflH, nagP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU07690

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides (according to Swiss-Prot)

• Protein family: peptidase M24A family (according to Swiss-Prot)

• Paralogous protein(s): Map

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34484

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yflG-yflH PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • YvoA: transcription repression (weak and not certain) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

CongHui You, HongYan Lu, Agnieszka Sekowska, Gang Fang, YiPing Wang, Anne-Marie Gilles, Antoine Danchin
The two authentic methionine aminopeptidase genes are differentially expressed in Bacillus subtilis.
BMC Microbiol: 2005, 5;57
[PubMed:16207374] [WorldCat.org] [DOI] (I e)