Difference between revisions of "CotY"

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* '''Description:''' spore coat protein (insoluble fraction) <br/><br/>
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* '''Description:''' spore coat protein (insoluble fraction), necessary for the assembly of the spre crust, the outermost layer of the spore coat <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein (insoluble fraction)
 
|style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein (insoluble fraction)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || resistance of the spore  
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|style="background:#ABCDEF;" align="center"|'''Function''' || spore coat assembly
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.857   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.857   
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=References=
 
=References=
  
<pubmed>19304857,7519271,15699190, 15621419</pubmed>
+
<pubmed>19304857,7519271,15699190, 15621419 20451384 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:22, 11 May 2010

  • Description: spore coat protein (insoluble fraction), necessary for the assembly of the spre crust, the outermost layer of the spore coat

Gene name cotY
Synonyms
Essential no
Product spore coat protein (insoluble fraction)
Function spore coat assembly
MW, pI 17 kDa, 4.857
Gene length, protein length 486 bp, 162 aa
Immediate neighbours cotZ, cotX
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CotY context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU11750

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: spore wall (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the mother cell (SigK) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Peter T McKenney, Adam Driks, Haig A Eskandarian, Paul Grabowski, Jonathan Guberman, Katherine H Wang, Zemer Gitai, Patrick Eichenberger
A distance-weighted interaction map reveals a previously uncharacterized layer of the Bacillus subtilis spore coat.
Curr Biol: 2010, 20(10);934-8
[PubMed:20451384] [WorldCat.org] [DOI] (I p)

Daniela Krajcíková, Magda Lukácová, Denisa Müllerová, Simon M Cutting, Imrich Barák
Searching for protein-protein interactions within the Bacillus subtilis spore coat.
J Bacteriol: 2009, 191(10);3212-9
[PubMed:19304857] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Ritsuko Kuwana, Takashi Okumura, Hiromu Takamatsu, Kazuhito Watabe
The ylbO gene product of Bacillus subtilis is involved in the coat development and lysozyme resistance of spore.
FEMS Microbiol Lett: 2005, 242(1);51-7
[PubMed:15621419] [WorldCat.org] [DOI] (P p)

J Zhang, H Ichikawa, R Halberg, L Kroos, A I Aronson
Regulation of the transcription of a cluster of Bacillus subtilis spore coat genes.
J Mol Biol: 1994, 240(5);405-15
[PubMed:7519271] [WorldCat.org] [DOI] (P p)