AlsD

• Description: acetolactate decarboxylase
  
  

Gene name	alsD
Synonyms
Essential	no
Product	acetolactate decarboxylase)
Function	overflow metabolism
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	28 kDa, 4.603
Gene length, protein length	765 bp, 255 aa
Immediate neighbours	ywrO, alsS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU36000

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (2S)-2-hydroxy-2-methyl-3-oxobutanoate = (3R)-3-hydroxybutan-2-one + CO₂ (according to Swiss-Prot)

• Protein family: alpha-acetolactate decarboxylase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed, in vitro phosphorylated by PrkC on Ser-88 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: Q04777

• KEGG entry: [3]

• E.C. number: 4.1.1.5

Expression and regulation

• Operon: alsS-alsD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induction by acetate (AlsR) PubMed
  • repressed as long as terminal electron acceptors are available for respiration (Rex) PubMed
  • subject to positive stringent control upon lysine starvation PubMed

Note: since acetate formation requires ackA activation by CcpA there is an indirect effect of CcpA on the alsSD operon: the operon is not expressed in ccpA mutants

• Regulatory mechanism:
  • stringent response: due to presence of adenines at +1 and +2 positions of the transcript PubMed
  • AlsR: transcription activation in the presence of acetate PubMed
  • Rex: transcription repression if the ratio NADH2/NAD is high PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP822, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: