Difference between revisions of "SacP"

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(Expression and regulation)
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=The gene=
 
=The gene=

Revision as of 15:05, 17 February 2010

Gene name sacP
Synonyms ipa-49d
Essential no
Product trigger enzyme: sucrose-specific
phosphotransferase system, EIIBC component
Function sucrose uptake and phosphorylation,
control of SacT activity
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 49 kDa, 7.026
Gene length, protein length 1383 bp, 461 aa
Immediate neighbours sacA, ywcJ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SacP context.gif
This image was kindly provided by SubtiList









The gene

Basic information

  • Locus tag: BSU38050

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)
  • Protein family: PTS permease, sucrose permease (Scr) family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression, purification of the EIIB domain in E. coli with N-terminal His-tag, in pWH844: pGP429, available in Stülke lab
    • for expression, purification of the EIIB domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP436, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References