Difference between revisions of "MccB"
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* '''Operon:''' ''[[yrrT]]-[[mtnN]]-[[mccA]]-[[mccB]]-[[yrhC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17056751 PubMed] | * '''Operon:''' ''[[yrrT]]-[[mtnN]]-[[mccA]]-[[mccB]]-[[yrhC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17056751 PubMed] | ||
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|16513748}} |
* '''Regulation:''' | * '''Regulation:''' | ||
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* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
** [[CymR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16513748 PubMed] | ** [[CymR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16513748 PubMed] | ||
| + | ** [[Spx]]: transcription activation {{PubMed|12642660}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
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=References= | =References= | ||
| − | <pubmed>17056751,16513748,, | + | <pubmed>17056751,16513748,, 12642660, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 19:13, 12 February 2010
- Description: cystathionine lyase/ homocysteine gamma-lyase
| Gene name | mccB |
| Synonyms | yrhB |
| Essential | no |
| Product | cystathionine lyase/ homocysteine gamma-lyase |
| Function | methionine-to-cysteine conversion |
| Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation | |
| MW, pI | 40 kDa, 5.215 |
| Gene length, protein length | 1137 bp, 379 aa |
| Immediate neighbours | yrhC, mccA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU27250
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: L-cystathionine + H2O = L-cysteine + NH3 + 2-oxobutanoate (according to Swiss-Prot)
- Protein family: trans-sulfuration enzymes family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O05394
- KEGG entry: [3]
- E.C. number: 4.4.1.1
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Marie-Françoise Hullo, Sandrine Auger, Olga Soutourina, Octavian Barzu, Mireille Yvon, Antoine Danchin, Isabelle Martin-Verstraete
Conversion of methionine to cysteine in Bacillus subtilis and its regulation.
J Bacteriol: 2007, 189(1);187-97
[PubMed:17056751]
[WorldCat.org]
[DOI]
(P p)
Sergine Even, Pierre Burguière, Sandrine Auger, Olga Soutourina, Antoine Danchin, Isabelle Martin-Verstraete
Global control of cysteine metabolism by CymR in Bacillus subtilis.
J Bacteriol: 2006, 188(6);2184-97
[PubMed:16513748]
[WorldCat.org]
[DOI]
(P p)
Shunji Nakano, Michiko M Nakano, Ying Zhang, Montira Leelakriangsak, Peter Zuber
A regulatory protein that interferes with activator-stimulated transcription in bacteria.
Proc Natl Acad Sci U S A: 2003, 100(7);4233-8
[PubMed:12642660]
[WorldCat.org]
[DOI]
(P p)
