Difference between revisions of "PriA"
| Line 1: | Line 1: | ||
| − | * '''Description:''' primosomal replication factor Y <br/><br/> | + | * '''Description:''' primosomal replication factor Y, part of the [[replisome]] <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 72: | Line 72: | ||
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' [[DnaD]]-[[PriA]], [[DnaB]]-[[PriA]] | + | * '''Interactions:''' [[DnaD]]-[[PriA]], [[DnaB]]-[[PriA]], part of the [[replisome]]: [[PolC]]-[[HolA]]-[[HolB]]-[[DnaX]]-[[DnaN]]-[[DnaG]]-[[DnaC]]-[[DnaI]]-[[DnaD]]-[[SsbA]]-[[DnaE]]-[[PriA]]-[[DnaB]] {{PubMed|20122408}} |
* '''Localization:''' | * '''Localization:''' | ||
| Line 120: | Line 120: | ||
=References= | =References= | ||
| − | <pubmed>17853894,,11585815, </pubmed> | + | <pubmed>17853894, 20122408,11585815, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:28, 4 February 2010
- Description: primosomal replication factor Y, part of the replisome
| Gene name | priA |
| Synonyms | yloJ |
| Essential | yes PubMed |
| Product | primosomal replication factor Y |
| Function | replication initiation |
| MW, pI | 91 kDa, 8.279 |
| Gene length, protein length | 2415 bp, 805 aa |
| Immediate neighbours | yloI, def |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU15710
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: helicase C-terminal domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions: DnaD-PriA, DnaB-PriA, part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
- Localization:
Database entries
- Structure:
- UniProt: P94461
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Glenn M Sanders, H Garry Dallmann, Charles S McHenry
Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases.
Mol Cell: 2010, 37(2);273-81
[PubMed:20122408]
[WorldCat.org]
[DOI]
(I p)
François Lecointe, Céline Sérèna, Marion Velten, Audrey Costes, Stephen McGovern, Jean-Christophe Meile, Jeffrey Errington, S Dusko Ehrlich, Philippe Noirot, Patrice Polard
Anticipating chromosomal replication fork arrest: SSB targets repair DNA helicases to active forks.
EMBO J: 2007, 26(19);4239-51
[PubMed:17853894]
[WorldCat.org]
[DOI]
(P p)
S Marsin, S McGovern, S D Ehrlich, C Bruand, P Polard
Early steps of Bacillus subtilis primosome assembly.
J Biol Chem: 2001, 276(49);45818-25
[PubMed:11585815]
[WorldCat.org]
[DOI]
(P p)
